Transformation and Galactose Induction | Homeworkmaven.com

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July 22, 2021
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Saccharomyces cerevisiae yph 252 transformation and galactose induction?

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for the first part of the question: if the plasmid has been verified after the amplification step in E. coli to assure that the insert has been correctly cloned (i.e. in frame with the tag, if you have any, and eventually sequenced to exclude any mutation that could have occurred during the PCR amplification) then the colony PCR on yeast is unnecessary. You should assume that each colony has been transformed with the plasmid (otherwise they would not grow on -ura plates). The colony PCR is used in case you want to integrate something in the genome (mainly to check deletion or endogenous tagging).

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