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Protocol for clonogenic survival assay for leukemia/ suspension cells?

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Assay

– count and plate cells in either 1x or 2x media the day prior ( I have tried both, neither was very effective. cell viability was definitely affected by 2x)

– mix 1:1 to reach optimal cell number and vigorously mix

– Plate in dishes with drugs

Alternate options I have tried have included using the 100ul/1mL technique

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