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Protocol for clonogenic survival assay for leukemia/ suspension cells?
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– count and plate cells in either 1x or 2x media the day prior ( I have tried both, neither was very effective. cell viability was definitely affected by 2x)
– mix 1:1 to reach optimal cell number and vigorously mix
– Plate in dishes with drugs
Alternate options I have tried have included using the 100ul/1mL technique
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