Measure of the Concentration | Homeworkmaven.com

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Is it necessary to measure the concentration of PCR/Gel purified product for A-tailing and TA cloning?

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in order to obtain optimal results for your ligation you should measure the insert concentration after gel-purification and calculate the amount of insert needed for a desired insert:vector ratio (5:1 to 3:1 should work in most cases). It depends on the molecular size of both vector and insert.

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