Band Size Explanation | Homeworkmaven.com

completed before 11 59 pm february 25th
July 22, 2021
physic question 4
July 22, 2021


There is a band of same size as that of my insert in the negative control PCR reaction, though it is much less intense than the other colonies. What could be the explanation?

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Solution Preview:

Best case scenario, you had a spillover from a neighboring well when loading your gel. To rule this out, load your negative control first & leave a few empty wells between the negative and the next sample.

 

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