antibiotic resistant bacteria | Homeworkmaven.com

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How to get countable colonies of a specific antibiotic resistant bacteria?

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First of all you have to isolate the bacterial colonies from xyz sample.

Gram staining is mandatory to differentiate the bacterial types whether they are gram positive or gram negative.

You can skip the identification of bacteria because you only have to count the number of resistant bacteria.

Purify your culture by doing 3-4 times quadrant streak. Now you’ll get a pure culture.

Use MHA (Muller Hinton agar) for antibiotic susceptibility (Kirby bauer method) of the cultures by using different antibiotic of your choice.

This makes you differentiate between the resistant and non resistant ones.

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